Pepsinogen I (PG I)

Anti- PG I antibody (Datasheet)

Source	Mouse monoclonal antibody，cultured in vitro
Platforms	Immunofluorescence, Chemiluminescence, Colloidal gold and Immunological latex turbidimetry
Catalog number	K50f4	K56t1
Applications	Capture	Detection
Buffer	1x PBS
Purity	Purity>98%，purified by Protein A/G chromatography
Storage instructions	Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

PG I protein (Datasheet)

Description	PG I protein (41.1kD) with His-tag, cultured in vitro
Applications	Calibrator and quality control product
Catalog number	K3916
Source	Mammalian cell
Purity	>90% as analysized by SDS-PAGE
Buffer	1 x PBS，pH 7.4
Storage instructions	Store at 2 - 8°C (35.6 – 46.4°F) for up to 3 years.
Sequence	IMYKVPLIRK KSLRRTLSER GLLKDFLKKH NLNPARKYFP QWEAPTLVDE QPLENYLDME YFGTIGIGTP AQDFTVVFDT GSSNLWVPSV YCSSLACTNH NRFNPEDSST YQSTSETVSI TYGTGSMTGI LGYDTVQVGG ISDTNQIFGL SETEPGSFLY YAPFDGILGL AYPSISSSGA TPVFDNIWNQ GLVSQDLFSV YLSADDQSGS VVIFGGIDSS YYTGSLNWVP VTVEGYWQIT VDSITMNGEA IACAEGCQAI VDTGTSLLTG PTSPIANIQS DIGASENSDG DMVVSCSAIS SLPDIVFTIN GVQYPVPPSA YILQSEGSCI SGFQGMNLPT ESGELWILGD VFIRQYFTVF DRANNQVGLA PVAHHHHHH
SDS-PAGE

Antibody Product Information

Antibody Evaluation (Immunofluorescence-based)

Immunofluorescence-Based test strip was prepared with K50f4-K56t1 antibody (30 Abbott calibrators, Concentration: 5-200 ng/ml, R² >97%)

Fig. 1 Clinical sample analysis

Accuracy
The quality control samples with concentration of 25 ng/ml-30 ng/ml and 70 ng/ml-80 ng/ml respectively were required to repeat test three times. B= (M-T) / T×100% (B: Relative Standard Deviation, M: Average, T: Concentration)

	Sample (ng/ml)	Test 1	Test 2	Test 3	Average (ng/ml)	RSD	Standard
Batch 1	26.59	23.54	25.78	28.43	25.92	-2.53%	±15%
Batch 1	78.34	77.23	75.98	79.12	77.44	-1.14%
Batch 2	26.59	25.22	24.27	25.8	25.10	-5.62%
Batch 2	78.34	79.69	78.94	75.34	77.99	-0.45%
Batch 3	26.59	27.89	27.94	27.2	27.68	4.09%
Batch 3	78.34	75.29	77.38	76.62	76.43	-2.44%

Repeatability
The quality control samples with concentration of 25 ng/ml-30 ng/ml and 70 ng/ml-80 ng/ml respectively were required to repeat test over 10 times. CV= (S / average

) ×100% (CV < 15% as indicated in the following table)

	Low Value （26.59ng/ml）	High Value （78.34ng/ml）
Test 1	27.12	82.01
Test 2	25.15	82.02
Test 3	27.98	76.40
Test 4	26.74	80.58
Test 5	29.18	75.67
Test 6	28.41	81.85
Test 7	24.24	81.94
Test 8	28.62	81.64
Test 9	27.70	85.61
Test 10	26.78	73.28
Average (ng/ml)	27.19	80.10
SD	1.55	3.75
CV	5.70%	4.68%
Standard	±15%

Antibody Evaluation (Immunological Turbidimetry)

Fig. 2 Calibration curve

R1: Buffer
R2: Combination of K50f4-K56t1 matched pair antibody and latex particals
Sample: PG I calibrator
Equipment: Hitachi 7080

Linear range

Linear dependence > 99% with concentration between 0-200 ng/ml.

Theoretical value (ng/ml)	Measured value 1	Measured value 2	Average
0.00	2.1	3.54	2.82
25.00	29.22	27.02	28.12
50.00	55.8	52.8	54.3
100.00	108.25	107.36	107.805
200.00	208.76	206.74	207.75

Fig. 3 Linearity range

Antibody Evaluation (Immunological Turbidimetry)

Fig. 4 Calibration curve

R1: Latex particals
R2: K50f4-K56t1 matched pair antibody
Sample: PG I calibrator
Equipment: Hitachi 7080

Description

胃蛋白酶原Ⅰ（PGⅠ）與胃蛋白酶原Ⅱ（PGⅡ）的分布情況

Pepsinogen I, the precursors of pepsin, is produced by the gastric mucosa and released into the gastric lumen and peripheral circulation. Pepsinogen consists of a single polypeptide chain of 375 amino acids with an average molecular weight of 42 kD. PG I (isoenzyme 1-5) is secreted mainly by chief cells in the fundic mucosa, whereas PG II (isoenzyme 6-7) is secreted by the pyloric glands and the proximal duodenal mucosa.

Precursor reflects numbers of stomach surface cells as well as glandular cells, and monitors gastric atrophy indirectly. They are also extraordinarily stable because they perform their jobs under the harsh conditions present in the digestive system. The atrophy of the corpus mucosa leads to low synthesis of pepsinogen I and hence its low release into the serum. Serum pepsinogen I indicates function and states of gastric mucosa.

Pepsinogen I (PG I)

Antibody Evaluation (Immunofluorescence-based)

Antibody Evaluation (Immunological Turbidimetry)

Linear range

Antibody Evaluation (Immunological Turbidimetry)

Related Products