Anti-Müllerian Hormone (AMH)
| Source |
Mouse monoclonal antibody, cultured in vitro |
| Catalog number |
K27n8 |
K25h6 |
K59a5 |
K60b6 |
| Applications |
Detection |
Capture |
Capture |
Detection |
| Platforms |
Immunofluorescence |
Chemiluminescence |
| Buffer |
1x PBS |
| Purity |
>98%，purified by Protein A/G chromatography |
| Storage instructions |
Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
Antibody Product Information
Antibody Evaluation (Immunofluorescence-based)
Immunofluorescence-Based test strip was prepared with K27n8-K25h6 matched antibody pairs (30 Beckman calibrators, Concentration: 0.5-30 ng/ml, R2>98%)
Fig. 1 Coincidence rate of Immunofluorescence-based sample
Accuracy
The quality control samples with concentration of 1.5 ng/ml-2.5 ng/ml and 15 ng/ml-25 ng/ml respectively were required to repeat test three times. B= (M-T) / T×100% (B: Relative Standard Deviation, M: Average, T: Concentration)
|
Sample
(ng/ml)
|
Test 1 |
Test 2 |
Test 3 |
Average
(ng/ml) |
RSD |
Standard |
| Batch 1 |
2.18 |
2.29 |
2.31 |
2.41 |
2.34 |
7.19% |
±15% |
| 21.29 |
23.15 |
22.51 |
23.97 |
23.21 |
9.02% |
| Batch 2 |
2.18 |
2.35 |
2.13 |
2.29 |
2.26 |
3.52% |
| 21.29 |
19.4 |
24.78 |
22.65 |
22.28 |
4.63% |
| Batch 3 |
2.18 |
2.39 |
2.38 |
2.34 |
2.37 |
8.72% |
| 21.29 |
23.24 |
20.55 |
22.72 |
22.17 |
4.13% |
Repeatability
The quality control samples with concentration of 1.5 ng/ml-2.5 ng/ml and 15 ng/ml-25 ng/ml respectively were required to repeat test over 10 times. CV= (S /
) ×100% (CV < 15% as indicated in the following table)
|
Low Value
（2.18ng/ml） |
High Value
（21.29ng/ml） |
| Test 1 |
2.09 |
25.36 |
| Test 2 |
2.06 |
24.17 |
| Test 3 |
2.55 |
23.65 |
| Test 4 |
2.52 |
25.17 |
| Test 5 |
2.11 |
25.18 |
| Test 6 |
2.33 |
21.36 |
| Test 7 |
2.41 |
20.91 |
| Test 8 |
2.39 |
26.55 |
| Test 9 |
2.05 |
21.19 |
| Test 10 |
2.47 |
23.87 |
| Average(ng/ml) |
2.30 |
23.74 |
| SD |
0.20 |
1.97 |
| CV |
8.72% |
8.31% |
| Standard |
±15% |
Description
Anti-Müllerian hormone (AMH) is a 140 kDa disulphide-linked homodimeric glycoprotein and a member of the transforming growth factor-β (TGF-β) superfamily of growth and differentiation factors. In the ovary, AMH has an inhibitory effect on primordial follicle recruitment and growth. It is also responsible for testicular development.
AMH tests may be used to evaluate ovarian reserve, monitor women who have certain types of ovarian cancer, help diagnose polycystic ovary syndrome (PCOS) and predict the start of menopause. AMH is the most sensitive component and the most accurate existing refection of ovarian reserve among the biomarkers. The ovarian reserve testing allows for tailored fertility treatment plans.
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