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C-reactive Protein (CRP)

product parametres

Anti-CRP antibody (Datasheet)

Description Mouse monoclonal antibody,cultured in vitro
Platforms Immunofluorescence, Colloidal gold and Immunological latex turbidimetry
Catalog number K1016 K1017
Applications Capture Detection
Linearity range 0.4-200 ug/ml (Colloidal gold), 0.1-200 ug/ml (Immunofluorescence)
Buffer 1 x PBS
Purity Purity>98%,purified by Protein A/G chromatography
Storage instructions Upon delivery aliquot and store at -80°C. Avoid freeze / thaw cycles.
product parametres

CRP protein (Datasheet)

Description Natural human CRP protein
Applications Calibrator and quality control product
Catalog number K1916
Purity >90% as analysized by SDS-PAGE
Buffer 1 x PBS,pH 7.4
Storage instructions Upon delivery aliquot and store at 2-8°C. Avoid freeze / thaw cycles.
Western-Blot western blot
Product Information

Antibody Product Information

Antibody Evaluation (Colloidal Gold-based)

The strip was prepared with capture antibody K1016 and marker antibody K1017. The serum samples were diluted into 0.4 ug/ml, 1.8 ug/ml, 6.63 ug/ml, 14.6 ug/ml, 22.9 ug/ml, 39.5 ug/ml, 57.1 ug/ml and 112 ug/ml. As indicated in Fig. 1, Color gradients were observed around T line.
Colloidal gold-based immunochromatographic test tripFig. 1 Colloidal gold-based immunochromatographic test strip
Antibody Evaluation (Immunofluorescence-based)
Immunofluorescence-Based test strip was prepared with K1016-K1017 matched antibody pairs (30 Beckman calibrators, Concentration: 0.1-220 ug/ml, R2>98%)
Coincidence rate of colloidal gold-based test strip

Fig. 2 Coincidence rate of Immunofluorescence-based test strip (K1016-K1017)

Accuracy
The quality control samples with concentration of 20 ug/ml-100 ug/ml and 1 ug/ml-10 ug/ml respectively were required to repeat test three times. B= (M-T) / T×100% (B: Relative Standard Deviation, M: Average, T: Concentration)
Sample
(ng/ml)
Test 1 Test 2 Test 3 Average
(ng/ml)
RSD Standard
Batch 1 9.84 10.95 9.19 10.46 10.20 3.66% ±15%
30.14 28.87 34.14 28.08 30.36 0.73%
Batch 2 9.84 9.08 10.94 8.88 9.63 -2.13%
30.14 30.47 26.62 27.55 28.21 -6.40%
Batch 3 9.84 10.40 10.77 8.50 9.89 0.51%
30.14 28.52 26.43 33.88 29.61 -1.76%
Repeatability
The quality control samples with concentration of 20 ug/ml-100 ug/ml and 1 ug/ml-10 ug/ml respectively were required to repeat test over 10 times. CV= (S /Average)×100% (CV < 15% as indicated in the following table)
Low Value
(9.84 ng/ml)
High Value
(30.14 ng/ml)
Test 1 10.20 33.93
Test 2 8.99 32.21
Test 3 10.08 34.44
Test 4 10.56 27.08
Test 5 9.79 32.14
Test 6 10.35 33.98
Test 7 9.12 30.08
Test 8 8.55 30.15
Test 9 9.40 33.72
Test 10 10.77 30.99
Average(μg/ml) 9.78 31.87
SD 0.738 2.328
CV 7.55% 7.30%
Standard ±15%
Antibody Evaluation (Immunological Turbidimetry)
Calibration curve

Fig. 3 Calibration curve

R1: Buffer

R2: Combination of K1016-K1017 matched antibody pairs and latex particals

Sample: CRP calibrator

Equipment: Hitachi 7080

Linear range
Linearity range

Fig. 4 Linearity range

Six calibrators were prepared using Latex particals and CRP antibody. Data show that Linear dependence > 0.99 with concentration between 0.1-200 ug/mL as indicated in Fig. 4

Clinical sample analysis
K1016-K1017 matched antibody pairs were employed to prepare kits to detect 60 clinical coincidence rate of CRP antibody. With R2 > 99%, the antibody can be used to develop CRP immunonephelometry materials.
CRP Clinical sample analysis

Fig. 5 Clinical sample analysis

Clinical Significance

Description

CRP is an acute-phase protein composed of five identical nonglycosylated 206 amino acid polypeptide subunits, which are noncovalently associated in a disc with cyclic pentameric symmetry. It plays an important role in protection against infection, clearance of damaged tissue, prevention of autoimmunity, and regulation of the inflammatory response as a non-specific marker.
As a non-specific marker of inflammation, CRP is considered to be directly involved in coronary plaque atherogenesis. Researches show that an elevated CRP level independently predicts adverse cardiac events at the primary and secondary prevention levels. It indicates that CRP is a useful prognostic indicator in patients with acute coronary syndrome, as elevated CRP levels are independent predictors of cardiac death, acute myocardial infarction, as well as congestive heart failure.
To determine a person's risk for heart disease, a more sensitive CRP test named a high-sensitivity C-reactive protein (hs-CRP) assay is available.
CRP is traditionally measured down to concentrations of 2-200 mg/L, whereas hs-CRP measures down to levels of 0.1-10 mg/L using latex Enhance immunoturbidimetric method. Full-range CRP assay can detect levels between 0.1-200 mg/l. This improved sensitivity allows full-range CRP to be used to detect low-grade inflammation.
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